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NEWS FLASH! LiveLongerLabs has filed multiple patents securing C60 all MCT (medium chain triglycerides) oils and curcumin, including, but not limited to olive, sunflower, coconut, black seed, and avocado. Stay tuned for exciting product developments.

These statements have not been evaluated by the Food and Drug Administration. These products are not intended to diagnose, treat, cure or prevent any disease.

 

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C60live RESEARCH

C60

World’s most efficient known free radical scavenger is C60 fullerene.

c60

34 oxygen radicals have been added onto a single C60 molecule.

c60

C60 fullerene is able to cross the blood-brain barrier.

c60

C60 fullerene derivatives are rapidly absorbed by tissues

LiveLongerLabs' Research Library
We research everything we do. Use SEARCH at the top of every page to find more information on the topic you'd like to read about.  If  you don't find your exact topic, visit PUBMED.GOV. This is where all medical research is published and openly shared.

Time Warp Experiment on Effects of Thinking and Youthfulness, by Harvard Psychologist, Ellen Langer

One day in the fall of 1981, eight men in their 70s stepped out of a van in front of a converted monastery in New Hampshire. They shuffled forward, a few of them arthritically stooped, a couple with canes. Then they passed through the door and entered a time warp. Perry Como crooned on a vintage radio. Ed Sullivan welcomed guests on a black-and-white TV. Everything inside — including the books on the shelves and the magazines lying around — were designed to conjure 1959. This was to be the men’s home for five days as they participated in a radical experiment, cooked up by a young psychologist named Ellen Langer.

The subjects were in good health, but aging had left its mark. “This was before 75 was the new 55,” says Langer, who is 67 and the longest-serving professor of psychology at Harvard. Before arriving, the men were assessed on such measures as dexterity, grip strength, flexibility, hearing and vision, memory and cognition — probably the closest things the gerontologists of the time could come to the testable biomarkers of age. Langer predicted the numbers would be quite different after five days, when the subjects emerged from what was to be a fairly intense psychological intervention.
Gathering the older men together in New Hampshire, for what she would later refer to as a counterclockwise study, would be a way to test this premise.

The men in the experimental group were told not merely to reminisce about this earlier era, but to inhabit it — to “make a psychological attempt to be the person they were 22 years ago,” she told me. “We have good reason to believe that if you are successful at this,” Langer told the men, “you will feel as you did in 1959.” From the time they walked through the doors, they were treated as if they were younger. The men were told that they would have to take their belongings upstairs themselves, even if they had to do it one shirt at a time.

Each day, as they discussed sports (Johnny Unitas and Wilt Chamberlain) or “current” events (the first U.S. satellite launch) or dissected the movie they just watched (“Anatomy of a Murder,” with Jimmy Stewart), they spoke about these late-'50s artifacts and events in the present tense — one of Langer’s chief priming strategies. Nothing — no mirrors, no modern-day clothing, no photos except portraits of their much younger selves — spoiled the illusion that they had shaken off 22 years.

At the end of their stay, the men were tested again. On several measures, they outperformed a control group that came earlier to the monastery but didn’t imagine themselves back into the skin of their younger selves, though they were encouraged to reminisce. They were suppler, showed greater manual dexterity and sat taller — just as Langer had guessed. Perhaps most improbable, their sight improved. Independent judges said they looked younger. The experimental subjects, Langer told me, had “put their mind in an earlier time,” and their bodies went along for the ride.
The results were almost too good. They beggared belief. “It sounded like Lourdes,” Langer said.

Though she and her students would write up the experiment for a chapter in a book for Oxford University Press called “Higher Stages of Human Development,” they left out a lot of the tantalizing color — like the spontaneous touch-football game that erupted between heretofore creaky seniors as they waited for the bus back to Cambridge. And Langer never sent it out to the journals. She suspected it would be rejected.

Various facts about how C60 acts in the body

C-60 is monomolecular – that is its one molecular thick.
C-60 shape can be thought of as that of a soccer ball wire frame with only the grid lines.
C-60 is unique in that the holes in the “wireframe” structure are large enough to allow hydrogen atoms to go through. Also, the node points on this grid can also have many hydrogen atoms attached to it.
Our bodies are mostly Carbon and Silicone and because of this characteristic, our bodies digest C-60 very well.
C-60 does no harm what’s so ever.
Our bodies do not break C-60 down but can excrete it. Once the C-60 gloms onto the hydrogen atoms in the body, it becomes a super, super antioxidant! They work at a nanomolecular level.
Because it is Carbon, it can pass through the cellular membranes and pass the blood-brain barrier. Other antioxidants, like Vitamin C, can’t do this the same way.
C-60 can cluster around the mitochondria of your cells, and the hydrogen globs onto the oxygen and thus instantly your oxidative stress damage to your body, the creation of which is known as Free Radicals, starts reducing. Free Radicals degrade our Telomeres, Free Radicals degrade our bodies. This degrading causes us to not replicate the cells as cleanly as before. 
C-60 wraps around the core of the cell and picks up all of the oxygen damage until it completely full of it and you excrete the C-60 with all of those toxins.
C-60 is 172 times more potent than Vitamin C as an antioxidant – Vitamin C is the most powerful antioxidant found on this planet… until the discovery of C-60 as a true Super Antioxidant. 

Proapoptotic and antimetastatic properties of supercritical CO2 extract of Nigella sativa Linn. against breast cancer cells.

Nigella sativa, commonly referred as black cumin, is a popular spice that has been used since the ancient Egyptians. It has traditionally been used for treatment of various human ailments ranging from fever to intestinal disturbances to cancer. This study investigated the apoptotic, antimetastatic, and anticancer activities of supercritical carbon dioxide (SC-CO2) extracts of the seeds of N. sativa Linn. against estrogen-dependent human breast cancer cells (MCF-7). Twelve extracts were prepared from N. sativa seeds using the SC-CO2 extraction method by varying pressure and temperature. Extracts were analyzed using FTIR and UV-Vis spectrometry. Cytotoxicity of the extracts was evaluated on various human cancer and normal cell lines. Of the 12 extracts, 1 extract (A3) that was prepared at 60°C and 2500 psi (~17.24 MPa) showed selective antiproliferative activity against MCF-7 cells with an IC50 of 53.34±2.15 μg/mL. Induction of apoptosis was confirmed by evaluating caspases activities and observing the cells under a scanning electron microscope. In vitro antimetastatic properties of A3 were investigated by colony formation, cell migration, and cell invasion assays. The elevated levels of caspases in A3 treated MCF-7 cells suggest that A3 is proapoptotic. Further nuclear condensation and fragmentation studies confirmed that A3 induces cytotoxicity through the apoptosis pathway. A3 also demonstrated remarkable inhibition in migration and invasion assays of MCF-7 cells at subcytotoxic concentrations. Thus, this study highlights the therapeutic potentials of SC-CO2 extract of N. sativa in targeting breast cancer.
 

Powdered black cumin seeds strongly improves serum lipids, atherogenic index of plasma and modulates anthropometric features in patients with Hashimoto's thyroiditis

BACKGROUND:

Hashimoto's thyroiditis is associated with serious alterations in serum lipids and glucose homeostasis. The aims of the current study were to evaluate the effect of powdered Nigella sativa on serum lipids, glucose homeostasis and anthropometric variables in patients with Hashimoto's thyroiditis.

 

METHODS:

Forty patients with Hashimoto's thyroiditis, aged between 22 and 50 years old, participated in the trial and were randomly allocated into two groups of intervention and control receiving powdered Nigella sativa or placebo daily for 8 weeks. Serum lipids, glucose homeostasis, and anthropometric variables were evaluated at baseline and after intervention.

 

RESULTS:

Treatment with Nigella sativa significantly reduced body weight and body mass index (BMI). Serum concentrations of low density lipoprotein cholesterol (LDL) and triglyceride (TG) also decreased in Nigella sativa-treated group after 8 weeks; while serum high density lipoprotein cholesterol (HDL) significantly increased after treatment with Nigella sativa (P < 0.05). None of these changes had been observed in placebo treated group. Serum Nesfatin-1 concentrations was in inverse relationship with serum triglyceride (TG) (r = - 0.31, P = 0.04).

 

CONCLUSIONS:

Giving attention to the potent beneficial effects of powdered black cumin seeds in improving serum lipid profile and anthropometric features in patients with Hashimoto's thyroiditis, this medicinal plant could be considered as a beneficial herbal supplement alongside with the disease- specific medications including Levothyroxine in management of Hashimoto's thyroiditis- related metabolic abnormalities.

Phytochemistry, pharmacology, and therapeutic uses of black seed (Nigella sativa)

Black seed (Nigella sativa) is an annual flowering plant from Ranunculaceae family, native to southwest Asia. This plant has many food and medicinal uses. he use of its seeds and oil is common for treatment of many diseases, including rheumatoid arthritis, asthma, inflammatory diseases, diabetes and digestive diseases.

The purpose of this study was to provide a comprehensive review on the scientific reports that have been published about N. sativa. The facts and statistics presented in this review article were gathered from the journals accessible in creditable databases such as Science Direct, Medline, PubMed, Scopus, EBSCO, EMBASE, SID and IranMedex. The keywords searched in Persian and English books on medicinal plants and traditional medicine, as well as the above reputable databases were "Black seed", "Nigella sativa", "therapeutic effect", and "medicinal plant". The results showed that N. sativa has many biological effects such as anti-inflammatory, anti-hyperlipidemic, anti-microbial, anti-cancer, anti-oxidant, anti-diabetic, anti-hypertensive, and wound healing activities. It also has effects on reproductive, digestive, immune and central nervous systems, such as anticonvulsant and analgesic activities. In summary, it can be used as a valuable plant for production of new drugs for treatment of many diseases.

Read research report on the PUBMED.gov, the US National Library of Medicine National Institutes of Health.
 

Effects of black seed (Nigella sativa) on metabolic parameters in diabetes mellitus: a systematic review.

BACKGROUND:

Current evidence indicated beneficial effects of some medicinal herbs on metabolic parameters. Nigella sativa is an example of herbs which can ameliorate metabolic factors in diabetes mellitus. Despite several narrative review studies on medicinal properties of NS, it seems that there is no systematic review to summarize effects of NS on glucose homoeostasis and lipid profile in diabetes mellitus. Therefore, the aim of present study was to review effects of N. sativa on metabolic parameters in diabetes mellitus.

 

RESULTS:

Finally 19 eligible articles (2 human trials, 14 animal models and 3 in vivo/in vitro studies) were selected. They indicated that N. sativa can modulate hyperglycemia and lipid profile dysfunction with various potential mechanisms including its antioxidant characteristics and effects on insulin secretion, glucose absorption, gluconeogenesis and gene expression. Some studies compared effects of various types (extract, oil, powdered) of N. sativa with each other and they reported different characteristics with various types of black seed.

A Review on Possible Therapeutic Effect of Nigella sativa and Thymoquinone in Neurodegenerative Diseases.

Medicinal plants have attracted great attention in the recent years and is increasingly applied instead of the chemical drugs. Several documents showed that herbal medicine traditionally and clinically applied in the cure and prevention of several diseases. In the recent years, different medicinal plants and their main components have been chosen in neurological therapy.

 

The less toxic effects, availability, and lower price of medicinal plants versus synthetic substances make them as excellent and simple selection in the treatment of nervous diseases. Nigella sativa (N. Sativa) L. (Ranunculaceae), well recognized as black cumin, has been utilized as a medicinal plant that has a strong traditional background. Thymoquinone (TQ) is one of the main active components of the volatile oil of N. sativa seeds and most effects and actions of N. Sativa are mainly related to TQ. The several pharmacological properties of N. sativa and TQ have been found, for example; anti-tumor, anti-microbial, anti-histaminic, immunomodulatory, anti-inflammatory, and anti-oxidant effects. Many reviews have investigated this valuable plant and its components, but none of them focused on their neuroprotective effects. Therefore, the aim of the present review was to show comprehensive and neuropharmacological properties of N. sativa and TQ. In this review, various studies on scientific databases regarding the effects of N. sativa and TQ in neurological diseases have been introduced. Studies on the neuroprotective effects of N. sativa and TQ which were published between1979 and 2018, were searched using various databases. The results of these studies showed that N. sativa and TQ have the protective effects against neurodegenerative diseases, including; Alzheimer, depression, encephalomyelitis, epilepsy, ischemia, Parkinson, and traumatic brain injury have been discussed in the cell lines and experimental animal models. Although there are many studies indicating the beneficial actions of this plant in the nervous system, the number of research projects relating to the human reports is rare.

Apoptotic Effect of Nigella sativa on Human Lymphoma U937 Cells

OBJECTIVE:

Nigella sativa is from botanical Ranunculaceae family and commonly known as black seed. Apoptotic effect of N. sativa and its apoptotic signaling pathways on U937 lymphoma cells are unknown.

 

MATERIALS AND METHODS:

In this study, we investigated selective cytotoxic and apoptotic effects of N. sativa extract and its apoptotic mechanisms on U937 cells. In addition, we also studied selective cytotoxic activity of thymoquinone that is the most active essential oil of N. sativa.

 

RESULTS:

Our results showed that N. sativa extract has selective cytotoxicity and apoptotic effects on U937 cells but not ECV304 control cells. However, thymoquinone had no significant cytotoxicity against on both cells. N. sativa extract increased significantly caspase-3, BAD, and p53 gene expressions in U937 cells.

CONCLUSIONS:

N. sativa may have anticancer drug potential and trigger p53-induced apoptosis in U937 lymphoma cells.

Natural therapeutic approach of Nigella sativa (Black seed) fixed oil in management of Sinusitis

Sinusitis is associated with inflammation and infections of air-filled cavities of sinuses. The aim of this study was to evaluate the potential efficacy of Nigella sativa seed fixed oil in management of sinusitis. The information was extracted from accessible international databases, traditional books, electronic resources, and unpublished data.

RESULTS:

The results of investigations on N. sativa seed fixed oil showed its therapeutic potential in treatment of sinusitis by its anti-inflammatory, antioxidant, antihistaminic, immune-modulator, antimicrobial and analgesic effects. The use of N. sativa seed fixed oil can inhibit the inflammation of sinuses and respiratory airways, microbial infections and finally help the patients suffering from clinical symptoms of sinusitis such as coryza, nasal congestion, headache, neck pain, earache and toothache. Clinical studies are required to evaluate its efficacy in patients with sinusitis in future.

Enhanced cytotoxicity and apoptosis by thymoquinone in combination with zoledronic acid in hormone- and drug-resistant prostate cancer cell lines.

PURPOSE:

Thymoquinone (TQ), an active ingredient of black seed oil (Nigella Sativa), has been shown to possess cytotoxic activity against a variety of cancer cell lines. Our purpose was to investigate if the cytotoxic and apoptotic effect of zoledronic acid (ZA) can be enhanced by the addition of the TQ in hormone- and drug-refractory prostate cancer cells PC-3 and DU-145.

RESULTS:

The combination of TQ and ZA resulted in a significant synergistic cytotoxic activity and DNA fragmentation when compared to any single agent alone, in a dose- and time-dependent manner. In addition, TQ and ZA combination increased the caspase 3/7 activity in PC-3 cell line, while this activity could not be demonstrated in DU-145 cell line.

CONCLUSION:

TQ and ZA had minimal hematological and non-hematological toxicity profile compared to cytotoxic agents. So, this combination may be an alternative approach for patients who are unable to be treated by conventional treatments because of poor performance status.

The clinical outcome of adjuvant therapy with black seed oil on intractable paediatric seizures: a pilot study

AIM:

To evaluate the effect of black seed oil, as add-on treatment to antiepileptic drugs (AEDs), on seizure frequency and severity as well as oxidative stress in intractable epilepsy patients.

 

METHODS:

A prospective, randomised, single-blinded, controlled, crossover pilot study. Five healthy children were included as controls. Thirty intractable epileptic children were randomly assigned to either Group I or II. Group I received placebo for four weeks, followed by a two-week washout period, and subsequently black seed oil for four weeks. Group II received the same intervention but in the reverse order. All patients received AEDs throughout the study period. Prior to allocation, all patients underwent a neurological assessment and evaluation of oxidative stress markers; total antioxidant capacity (TAC) and malondialehyde (MDA). Patients were assessed at Weeks 4 and 10 for oxidative stress markers and seizure frequency and severity.

 

RESULTS:

At baseline, both groups (I, II) had significantly lower serum TAC levels relative to healthy controls (p=0.007), while MDA levels were unchanged. After the 4-week period of black seed oil administration, there was no significant difference between the two groups with regards to seizure frequency, severity, or oxidative stress markers (TAC and MDA; p>0.05). Eight patients had >50% reduction in seizure frequency/severity after black seed oil versus placebo.

 

CONCLUSION:

Children with intractable epilepsy show evidence of oxidative stress. Administration of 40-80 mg/kg/day of black seed oil as add-on therapy did not alter either oxidative stress markers or seizure frequency or severity in intractable epileptic patients.

Therapeutic value of black seed oil in methotrexate hepatotoxicity in Egyptian children with acute lymphoblastic leukemia

'Acute lymphoblastic leukemia is the most common childhood malignancy'. Adding methotrexate to treatment protocols increased survival rate in children with leukemia. Methotrexate efficacy is limited by its hepatotoxicity.

AIM OF THE STUDY:

To assess the therapeutic value of Black seed oil in 'methotrexate induced hepatotoxicity in Egyptian children with acute lymphoblastic leukemia'.

 

SUBJECTS AND METHODS:

This study was conducted on 40 children with acute lymphoblastic leukemia' including 20 patients under methotrexate therapy and Black seeds 80 mg/kg/day for one week after each methotrexate dose [Group II] and 20 patients under methotrexate therapy and placebo [Group III]. This study included also '20 healthy children of matched age and sex as a control group' [Group I]. All patients were subjected to complete blood picture, bone marrow aspiration and liver functions.

 

RESULTS:

No significant differences in liver functions between group II and III before therapy were observed. There were nonsignificant increase in total, direct and indirect serum bilirubin, serum ALT, AST, and alkaline phosphatase levels and prothrombin time in group II after methotrexate and Black seed oil therapy but there was significant increase in group III after treatment with methotrexate and placebo with 'significant differences between group II and III ' after therapy. There were significant differences in prognosis regarding remission, relapse, death and 'disease free survival but no significant difference in overall survival between group II and III'.

 

CONCLUSION:

Black cumin seeds decreased MTX hepatotoxicity and improved survival in children with ALL and can be recommended as adjuvant drug in patients with ALL under methotrexate therapy.

Anti-neoplastic agent thymoquinone induces degradation of α and β tubulin proteins in human cancer cells without affecting their level in normal human fibroblasts

The microtubule-targeting agents derived from natural products, such as vinca-alkaloids and taxanes are an important family of efficient anti-cancer drugs with therapeutic benefits in both haematological and solid tumors. These drugs interfere with the assembly of microtubules of α/β tubulin heterodimers without altering their expression level. The aim of the present study was to investigate the effect of thymoquinone (TQ), a natural product present in black cumin seed oil known to exhibit putative anti-cancer activities, on α/β tubulin expression in human astrocytoma cells (cell line U87, solid tumor model) and in Jurkat cells (T lymphoblastic leukaemia cells). TQ induced a concentration- and time-dependent degradation of α/β tubulin in both cancer cell types. This degradation was associated with the up-regulation of the tumor suppressor p73 with subsequent induction of apoptosis. Interestingly, TQ had no effect on α/β tubulin protein expression in normal human fibroblast cells, which were used as a non-cancerous cell model. These data indicate that TQ exerts a selective effect towards α/β tubulin in cancer cells. In conclusion, the present findings indicate that TQ is a novel anti-microtubule drug which targets the level of α/β tubulin proteins in cancer cells. Furthermore, they highlight the interest of developing anti-cancer therapies that target directly tubulin rather than microtubules dynamics.

Anticancer activity of Nigella sativa (black seed) - a review

Nigella sativa (N. sativa) seed has been an important nutritional flavoring agent and natural remedy for many ailments for centuries in ancient systems of medicine, e.g. Unani, Ayurveda, Chinese and Arabic Medicines. Many active components have been isolated from N. sativa, including thymoquinone, thymohydroquinone, dithymoquinone, thymol, carvacrol, nigellimine-N-oxide, nigellicine, nigellidine and alpha-hederin. In addition, quite a few pharmacological effects of N. sativa seed, its oil, various extracts and active components have been identified to include immune stimulation, anti-inflammation, hypoglycemic, antihypertensive, antiasthmatic, antimicrobial, antiparasitic, antioxidant and anticancer effects. Only a few authors have reviewed the medicinal properties of N. sativa and given some description of the anticancer effects. A literature search has revealed that a lot more studies have been recently carried out related to the anticancer activities of N. sativa and some of its active compounds, such as thymoquinone and alpha-hederin. Acute and chronic toxicity studies have recently confirmed the safety of N. sativa oil and its most abundant active component, thymoquinone, particularly when given orally. The present work is aimed at summarizing the extremely valuable work done by various investigators on the effects of N. sativa seed, its extracts and active principles against cancer. Those related to the underlying mechanism of action, derivatives of thymoquinone, nano thymoquinone and combinations of thymoquinone with the currently used cytotoxic drugs are of particular interest. We hope this review will encourage interested researchers to conduct further preclinical and clinical studies to evaluate the anticancer activities of N. sativa, its active constituents and their derivatives.

A review on therapeutic potential of Nigella sativa: A miracle herb

Medicinal plants have been used for curing diseases for many centuries in different indigenous systems of medicine as well as folk medicines... Many researchers are focusing on medicinal plants since only a few plant species have been thoroughly investigated for their medicinal properties, potential, mechanism of action, safety evaluation and toxicological studies.

 

Among various medicinal plants, Nigella sativa (N. sativa) (Family Ranunculaceae) is emerging as a miracle herb with a rich historical and religious background since many researches revealed its wide spectrum of pharmacological potential. N. sativa is commonly known as black seed. N. sativa is native to Southern Europe, North Africa and Southwest Asia and it is cultivated in many countries in the world like Middle Eastern Mediterranean region, South Europe, India, Pakistan, Syria, Turkey, Saudi Arabia[1].

The seeds of N. sativa and their oil have been widely used for centuries in the treatment of various ailments throughout the world. And it is an important drug in the Indian traditional system of medicine like Unani and Ayurveda[2],[9]. Among Muslims, it is considered as one of the greatest forms of healing medicine available due to it was mentioned that black seed is the remedy for all diseases except death in one of the Prophetic hadith. It is also recommended for use on regular basis in Tibb-e-Nabwi (Prophetic Medicine)[3]...

Read the extensive report, which includes a review of Black Seed's Antibacterial activity, Antifungal activity, Anti-schistosomiasis activity, Antioxidant activity, Antidiabetic activity, Anticancer activity, Anti-inflammatory and analgesic activity, Immunomodulatory activity, Cardiovascular activity, Gastro-protective activity, Hepato-protective activity, Nephroprotective activity, Pulmonary-protective activity and anti-asthmatic effects, Testicular-protective activity, Neuro-pharmacolgical activities, Anticonvulsant activity, Contraceptive and anti-fertility activity, Antioxytocic activity, and Toxicological studies.

9 Proven Black Seed Oil Benefits that Boost Your Health, By Dr. Josh Axe, DC, DMN, CNS

If you take a look at the hundreds of scientific peer-reviewed articles that have been published about black seed oil benefits, one fact is clear: There are few issues that it cannot help the body overcome. With virtually no side effects, the healing prowess of black seed oil — back from black cumin seeds — is actually quite unbelievable, and it boggles the mind that most people have never even heard of it!

Read on find out what black seed oil is all about, along with all the wonderful black seed oil benefits out there.

Thymoquinone induces apoptosis in bladder cancer cell via endoplasmic reticulum stress-dependent mitochondrial pathway.

BACKGROUND:

Thymoquinone (TQ), the major active compound isolated from black seed oil (Nigella sativa), has been reported to exhibit anti-inflammatory and anticancer abilities. However, the exact molecular mechanism underlying the anticancer effect of TQ is still poorly understood, especially in regard to TQ's effect on endoplasmic reticulum stress-mediated apoptosis.

KEY FINDING:

TQ has a significant cytotoxicity on bladder cancer cells and can inhibit their proliferation and induce apoptosis. The protein changes of Bcl-2, Bax, cytochrome c and endoplasmic reticulum stress-related proteins (GRP78, CHOP, and caspase-12) revealed that the anticancer effect of TQ was associated with mitochondrial dysfunction and the endoplasmic reticulum stress pathway. Pretreatment with a pan-caspase inhibitor, Z-VAD-fmk, or an ER stress inhibitor, 4-PBA, or knockdown of CHOP by shRNA can partly reverse the pro-apoptotic effect of TQ by enhancing the expression of the anti-apoptotic protein Bcl-2, blocking the release of cytochrome c and the translocation of Bax from the cytoplasm to mitochondria.

 

SIGNIFICANCE:

Our findings provide the first demonstration of the anticancer effect of TQ on bladder cancer, and the relationship between ER stress and mitochondrial dysfunction was clearly understood when the apoptosis progressed is revealed.

Anti-Aging Effect of Nigella Sativa Fixed Oil on D-Galactose-Induced Aging in Mice

OBJECTIVES:

Aging is an unconscious and gradual process that can lead to changes in biological systems. Induction of oxidative stress and apoptosis, hepatotoxicity and neurotoxicity are involved in the aging process. Regarding the antioxidant property of black seed oil, the aim of this study was to evaluate the anti-aging effect of Nigella sativa (N. sativa) oil on d-galactose-induced aging in mice.

 

RESULTS:

Administration of D-galactose (500 mg/kg, SC) for 42 days increased serum levels of ALT and AST, as well as the MDA content, in brain and liver tissues, but decreased the GSH content. Additionally, the levels of apoptotic proteins, including Bax, procaspase-3 and caspase-3 cleaved, were markedly increased. N. sativa oil (0.1 and 0.2 mL/kg) diminished the levels of the biochemical markers ALT and AST. Administration of black seed oil (0.1, 0.2 and 0.5 mL/kg) reduced lipid peroxidation and at doses 0.1 and 0.2 mL/kg significantly recovered the GSH content. The oil decreased Bax/Bcl2 levels and at 0.1 mL/kg down-regulated the expressions of caspase-3 (pro and cleaved) proteins in brain and liver tissues.

 

CONCLUSION:

Through its antioxidant and anti-apoptosis properties, black seed oil exhibited an anti-aging effect in a model of aging induced with D-galactose.

Finding Novel Antibiotic Substances from Medicinal Plants - Antimicrobial Properties of Nigella Sativa Directed against Multidrug-resistant Bacteria

The progressive rise in multidrug-resistant (MDR) bacterial strains poses serious problems in the treatment of infectious diseases. While the number of newly developed antimicrobial compounds has greatly fallen, the resistance of pathogens against commonly prescribed drugs is further increasing. This rise in resistance illustrates the need for developing novel therapeutic and preventive antimicrobial options. The medicinal herb Nigella sativa and its derivatives constitute promising candidates. In a comprehensive literature survey (using the PubMed data base), we searched for publications on the antimicrobial effects of N. sativa particularly directed against MDR bacterial strains. In vitro studies published between 2000 and 2015 revealed that N. sativa exerted potent antibacterial effects against both Gram-positive and Gram-negative species including resistant strains. For instance, N. sativa inhibited the growth of bacteria causing significant gastrointestinal morbidity such as Salmonella, Helicobacter pylori, and Escherichia coli. However, Listeria monocytogenes and Pseudomonas aeruginosa displayed resistance against black cumin seed extracts. In conclusion, our literature survey revealed potent antimicrobial properties of N. sativa against MDR strains in vitro that should be further investigated in order to develop novel therapeutic perspectives for combating infectious diseases particularly caused by MDR strains.

Induction of apolipoprotein A-I gene expression by black seed (Nigella sativa) extracts

CONTEXT:

Black seed [Nigella sativa L. (Ranunculaceae)] has been shown in animal models to lower serum cholesterol levels.

 

OBJECTIVES:

In order to determine if extracts from black seed have any effects on high-density lipoprotein (HDL), we characterized the effects of black seed extract on apolipoprotein A-I (apo A-I) gene expression, the primary protein component of HDL.

RESULTS:

Extracts from black seeds significantly increased hepatic and intestinal apo A-I secretion, as well as apo A-I mRNA and gene promoter activity. This effect required a PPARα binding site in the apo A-I gene promoter. Treatment of the extract with either heat or trypsin had no effect on its ability to induce apo A-I secretion. Treatment with black seed extract induced PPARα expression 9-fold and RXRα expression 2.5-fold. Furthermore, the addition of PPARα siRNA but not a control siRNA prevented some but not all the positive effects of black seed on apo A-I secretion.

 

DISCUSSION:

Black seed extract is a potent inducer of apo A-I gene expression, presumably by enhancing PPARα/RXRα expression.

 

CONCLUSIONS:

We conclude that black seed may have beneficial effects in treating dyslipidemia and coronary heart disease.

Using Black Seed Oil To Treat Cancer

Thymoquinone has been shown to modulate nine out of the ten hallmarks of cancer. Experts are urging more research into thymoquinone, which is the active main constituent in black seed oil, for adjuvants to pharmaceutical cancer treatments, and signalling pathways that black cumin seed oils play in being a cancer killer.

Cancer depending on what form of cancer, in the most basic explanation is cells gone wrong, the cause of is most often not discovered of which malignant cancers are the ones to be most worried about.

 

Many studies have shown the effects of Nigella sativa on various different cancer cells, and the list is long offering up over 57 reviewed papers on the subject finding black cumin to be effective against cancers such as blood, breast, colon, pancreatic, lung, leukemia, skin, fibrosarcoma, renal, prostate, and cervical cancer.

16 More Reasons Black Seed is 'The Remedy For Everything But Death'

Known since ancient times as a 'remedy for everything but death,' an increasingly vast body of scientific research reveals that it is indeed one of nature's most potent and versatile healing agents.
A year ago, we wrote an article about nigella sativa (aka black seed) titled, 'The Remedy For Everything But Death.' It described the research on the many ways in which black seed (nigella sativa) is a potentially life-saving medicinal food, and is one of our most popular articles, with over 700K social media shares. Read that entire article @ GreenMedInfo.com. https://www.greenmedinfo.com/blog/black-seed-remedy-everything-death

The article summarized 10 of the seed's remarkable health benefits:

Type 2 Diabetes

Helicobacter Pylori Infection

Epilepsy

High Blood pressure

Asthma

Acute tonsillopharyngitis

Chemical Weapons Injury

Colon Cancer

MRSA

Opiate Addiction/Withdrawal
 

Since then, the biomedical research on black seed has continued to flourish, with another 78 studies published and cited on the National Library of Medicine's biomedical database MEDLINE over the past 11 months.

Here are 16 additional potential health benefits to add to the growing list:

  1. Prevents Radiation Damage: Nigella sativa oil (NSO) and its active component, thymoquinone, protect brain tissue from radiation-induced nitrosative stress.[i]

  2. Protects Against Damage from Heart Attack: A thymoquinone extract from nigella sativa has a protective effect against damage associated with experimental heart attack.[ii]

  3. Prevents Morphine Dependence/Toxicity: An alcohol extract of nigella sativa reduces morphine-associated conditioned place preference, an indication of morphine intoxication, dependence and tolerance.[iii]

  4. Prevents Kidney Damage Associated with Diabetes: A thymoquinone extract from nigella sativa has protective effects on experimental diabetic nephropathy.[iv]

  5. Prevents Post-Surgical Adhesions: Covering peritoneal surfaces with Nigella sativa oil (NSO) after peritoneal trauma is effective in decreasing peritoneal adhesion formation in an experimental model.[v]

  6. Prevents Alzheimer's Associated Neurotoxicity: A thymoquinone extract from nigella sativa has protective effects on experimental diabetic prevents neurotoxicity and Aβ1-40-induced apoptosis in the cell model.[vi]

  7. Suppresses Breast Cancer Growth: : A thymoquinone extract from nigella sativa inhibits tumor growth and induces programmed cell death (apoptosis) in a breast cancer xenograft mouse model.[vii] [viii]

  8. Exhibits Anti-Psoriasis Properties: The alcohol extract of nigella sativa seeds exhibit anti-psoriatic activity, consistent with its medicinal use in traditional medicine.[ix]

  9.  Prevents Brain Pathology Associated with Parkinson's Disease: A thymoquinone extract from nigella sativa protects cultured neurons against αSN-induced synaptic toxicity, a pathology observed in the brains of patients with Parkinson's disease and dementia with Lewy bodies.[x]

  10.  Kills Highly Aggressive Gliobastoma Brain Cancer Cells: A thymoquinone extract from nigella sativa exhibits glioblastoma cell killing activity. [xi]

  11.  Kills Leukemia Cells: A thymoquinone from nigella sativa induces mitochondria-mediated apoptosis in acute lymphoblastic leukaemia in vitro.[xii]

  12.  Suppresses Liver Cancer Growth: A thymoquinone extract from nigella sativa prevents chemically-induced cancer in a rat model.[xiii]

  13.  Prevents Diabetic Pathologies: A water and alcohol extract of nigella sativa at low doses has a blood-sugar lowering effect and ameliorative effect on regeneration of pancreatic islets, indicating its value as a therapeutic agent in the management of diabetes mellitus.[xiv]

  14. Suppresses Cervical Cancer Cell Growth: A thymoquinone extract from nigella sativa exhibits anti-proliferative, apoptotic and anti-invasive properties in a cervical cancer cell line.[xv]

  15.  Prevents Lead-Induced Brain Damage: A thymoquinone extract from nigella sativa ameliorates lead-induced brain damage in Sprague Dawley rats.[xvi]

  16. Kills Oral Cancer Cells: A thymoquinone extract from nigella sativa induces programmed cell death (apoptosis) in oral cancer cells.[xvii]

Black Seed - 'The Remedy for Everything but Death'

The seeds of the annual flowering plant, Nigella Sativa, have been prized for their healing properties since time immemorial. While frequently referred to among English-speaking cultures as Roman coriander, black sesame, black cumin, black caraway and onion seed, it is known today primarily as black seed, which is at the very least an accurate description of its physical appearance. The earliest record of its cultivation and use come from ancient Egypt.

Black seed oil, in fact, was found in Egyptian pharoah Tutankhamun's tomb, dating back to approximately 3,300 years ago.[i] In Arabic cultures, black cumin is known as Habbatul barakah, meaning the "seed of blessing." It is also believed that the Islamic prophet Mohammed said of it that it is "a remedy for all diseases except death."

 

Benefits of Black Seed

Many of black cumin's traditionally ascribed health benefits have been thoroughly confirmed in the biomedical literature. In fact, since 1964, there have been 656 published, peer-reviewed studies referencing it.

We have indexed salient research, available to view on GreenMedInfo.com on our Black Seed (Nigella Sativa) page, on well over 40 health conditions that may be benefited from the use of the herb, including over 20 distinct pharmacological actions it expresses, such as:

  • Analgesic (Pain-Killing)

  • Anti-Bacterial

  • Anti-Inflammatory

  • Anti-Ulcer

  • Anti-Cholinergic

  • Anti-Fungal

  • Ant-Hypertensive

  • Antioxidant

  • Antispasmodic

  • Antiviral

  • Bronchodilator

  • Gluconeogenesis Inhibitor (Anti-Diabetic)

  • Hepatoprotective (Liver Protecting)

  • Hypotensive

  • Insulin Sensitizing

  • Interferon Inducer

  • Leukotriene Antagonist

  • Renoprotective (Kidney Protecting)

  • Tumor Necrosis Factor Alpha Inhibitor

 

The article summarized 10 of the seed's remarkable health benefits:

Type 2 Diabetes

Helicobacter Pylori Infection

Epilepsy

High Blood pressure

Asthma

Acute tonsillopharyngitis

Chemical Weapons Injury

Colon Cancer

MRSA

Opiate Addiction/Withdrawal

Effect of Nigella sativa seed administration on prevention of febrile neutropenia during chemotherapy among children with brain tumors

PURPOSE:

Seeds of Nigella sativa (NS) are used to combat various disease conditions through their antibacterial effects. To evaluate the seeds' potential, we studied their effect on the prevention of febrile neutropenia (FN) in children with brain tumors.

METHODS:

A randomized pretest-post-test control group study including 80 children (2-18 years) with brain tumors undergoing chemotherapy were equally allocated into two groups. Intervention group received 5 g of NS seeds daily throughout treatment while controls received nothing. CBC with differentials, incidence of FN, and LOS were noted on each follow-up.

 

RESULTS:

The majority of children 38/40 (95%), of the intervention group, took the seeds for 3-9 consecutive months. Eight out of 372 (2.2%) FN episodes were experienced by children of intervention group compared to controls 63/327 (19.3%) (p = 0.001) and a shorter LOS (median = 2.5 days) vs 5 days in the control group (p = 0.006). Children in both groups belonged to almost same geographical area with similar socio-economic background. Weights of children were almost equal at diagnosis.

 

CONCLUSION:

NS seeds showed a decrease in incidence of FN in children with brain tumors with shortening of subsequent LOS which may improve their outcome and thereby quality of life. Larger scale studies are needed to further evaluate the seeds' potential.

Effect of Nigella sativa seed administration on prevention of febrile neutropenia during chemotherapy among children with brain tumors

PURPOSE:

Seeds of Nigella sativa (NS) are used to combat various disease conditions through their antibacterial effects. To evaluate the seeds' potential, we studied their effect on the prevention of febrile neutropenia (FN) in children with brain tumors.

METHODS:

A randomized pretest-post-test control group study including 80 children (2-18 years) with brain tumors undergoing chemotherapy were equally allocated into two groups. Intervention group received 5 g of NS seeds daily throughout treatment while controls received nothing. CBC with differentials, incidence of FN, and LOS were noted on each follow-up.

RESULTS:

The majority of children 38/40 (95%), of the intervention group, took the seeds for 3-9 consecutive months. Eight out of 372 (2.2%) FN episodes were experienced by children of intervention group compared to controls 63/327 (19.3%) (p = 0.001) and a shorter LOS (median = 2.5 days) vs 5 days in the control group (p = 0.006). Children in both groups belonged to almost same geographical area with similar socio-economic background. Weights of children were almost equal at diagnosis.

CONCLUSION:

NS seeds showed a decrease in incidence of FN in children with brain tumors with shortening of subsequent LOS which may improve their outcome and thereby quality of life. Larger scale studies are needed to further evaluate the seeds' potential.

Review on Clinical Trials of Black Seed (Nigella sativa ) and Its Active Constituent, Thymoquinone

BACKGROUND:

A significant role in pathogenesis of cholangitis is attributed to excessive reactive oxygen species production and oxidative stress. Therefore, antioxidants could be promising therapeutics.

 

AIMS:

The effects of powerful free radical scavenger C60 fullerene on hepatic and pancreatic manifestations of acute and chronic cholangitis in rats were aimed to be discovered.

RESULTS:

On AC, C60FAS normalized elevated bilirubin, alkaline phosphatase, and triglycerides, diminished fibrotic alterations in liver, and improved pancreas state when applied by both ways. Additionally, C60FAS per os significantly reduced the signs of inflammation in liver and pancreas. On CC, C60FAS also mitigated liver fibrosis and inflammation, improved pancreas state, and normalized alkaline phosphatase and triglycerides. The remedy effect of C60FAS was more expressed compared to that of prednisolone on both models. Furthermore, C60FAS inhibited pan-cytokeratin expression in HepG2 cells in a dose-dependent manner.

 

CONCLUSION:

Pristine C60 fullerene inhibits liver inflammation and fibrogenesis and partially improved liver and pancreas state under acute and chronic cholangitis.

Review on Clinical Trials of Black Seed (Nigella sativa ) and Its Active Constituent, Thymoquinone

Nigella sativa (black seed or black cumin), which belongs to the Ranunculacea family, is an annual herb with many pharmacological properties. Among its many active constituents, thymoquinone (TQ) is the most abundant constituent of the volatile oil of Nigella sativa (N. sativa) seeds, and it is the constituent to which most properties of this herb are attributed.

Methods

PubMed-Medline, Scopus, and Web of Science databases were searched to identify randomized control trials (RCTs) investigating the therapeutic effects of N. sativa and/or TQ. In this review, we investigated the clinical uses of N. sativa and TQ in the prevention and the treatment of different diseases and morbidity conditions in humans.

 

Results

Black seed and TQ are shown to possess multiple useful effects for the treatment of patients with several diseases, such as inflammatory and auto-immune disorders, as well as metabolic syndrome. Also, other advantages, including antimicrobial, anti-nociceptive and anti-epileptic properties, have been documented. The side effects of this herbal medicine appear not to be serious, so it can be applied in clinical trials because of its many advantages.

 

Conclusion

Some effects of N. sativa, such as its hypoglycemic, hypolipidemic and bronchodilatory effects, have been sufficiently studied and are sufficiently understood to allow for the next phase of clinical trials or drug developments. However, most of its other effects and applications require further clinical and animal studies.

Novel therapeutic approaches of natural oil from black seeds and its underlying mechanisms against kidney dysfunctions in haloperidol-induced male rats

BACKGROUND:

Antipsychotic drugs could be nephrotoxic in schizophrenia patients.

 

METHODS:

The present study investigated the protective effect of oil from black seed on kidney dysfunctions using several biological approaches in adult rats. The animals were divided into six groups (n=10): normal control rats; haloperidol (HAL)-induced rats: induced rats were pre-, co- and post-treated with black seed oil (BSO), respectively, and the last group was treated with the oil only. The treatment was done through oral administration, and the experiment lasted 14 days.

 

RESULTS:

Therapeutic administration of HAL to rats caused reduction in both enzymatic and non-enzymatic proteins mediated by stable OH˙ and DPPH free radicals. K+, Na+ and MDA contents as well as 51 nucleotidase, aldose-reductase activities were increased with corresponding decrease in the activity of lactate dehydrogenase (LDH) in HAL-induced toxicity rats. Contrariwise, differential treatments with BSO prevented and reversed the nephrotoxicity by depleting K+, Na+, MDA contents and aldose-reductase activity, and AMP hydrolysis with increased adenosine triphosphate (ATP) in the PMFs of rat kidney. The cytotoxicity of HAL elicited on both inner renal cortex and outer medulla was equally alleviated by combined active molecules of oil from black seed (OBS). However, pre-, co- and post-treatment demonstrate significant approaches in averting nephrotoxicity of neuroleptic drug (HAL) via several biological mechanisms.

 

CONCLUSIONS:

This study therefore validates the use of black seed oil as therapy particularly for individuals with renal dysfunctions.

​What Are Free Radicals?

Oxidative stress occurs when an oxygen molecule splits into single atoms with unpaired electrons, which are called free radicals.


The body is under constant attack from oxidative stress. Oxygen in the body splits into single atoms with unpaired electrons. Electrons like to be in pairs, so these atoms, called free radicals, scavenge the body to seek out other electrons so they can become a pair. This causes damage to cells, proteins and DNA. 


Free radicals are associated with human disease, including cancer, atherosclerosis, Alzheimer's disease, Parkinson's disease and many others. They also may have a link to aging, which has been defined as a gradual accumulation of free-radical damage, according to Christopher Wanjek, the Bad Medicine columnist for Live Science.

Oxidative Stress in Chronic Obstructive Pulmonary Disease

Oxidative Stress in Chronic Obstructive Pulmonary Disease

 

American Journal of Respiratory and Critical Care Medicine

JOHN E. REPINE , AALT BAST , IDA LANKHORST , and The Oxidative Stress Study Group

 

Chronic obstructive pulmonary disease (COPD) is a major worldwide health problem that has an increasing prevalence and mortality (12). Oxidative stress, which can be defined as an increased exposure to oxidants and/or decreased antioxidant capacities, is widely recognized as a central feature of many diseases (34). Considerable evidence now links COPD with increased oxidative stress (56). The purpose of this review is to describe the role and origin of the oxidant–antioxidant disturbances that participate in the development of COPD. Our presentation also addresses ways of assessing the contribution of oxidants and identifies therapeutic approaches that could improve cellular oxidant–antioxidant balance in the lungs of COPD patients.

Effects of Pristine C60 Fullerenes on Liver and Pancreas in α-Naphthylisothiocyanate-Induced Cholangitis

BACKGROUND:

A significant role in pathogenesis of cholangitis is attributed to excessive reactive oxygen species production and oxidative stress. Therefore, antioxidants could be promising therapeutics.

 

AIMS:

The effects of powerful free radical scavenger C60 fullerene on hepatic and pancreatic manifestations of acute and chronic cholangitis in rats were aimed to be discovered.

RESULTS:

On AC, C60FAS normalized elevated bilirubin, alkaline phosphatase, and triglycerides, diminished fibrotic alterations in liver, and improved pancreas state when applied by both ways. Additionally, C60FAS per os significantly reduced the signs of inflammation in liver and pancreas. On CC, C60FAS also mitigated liver fibrosis and inflammation, improved pancreas state, and normalized alkaline phosphatase and triglycerides. The remedy effect of C60FAS was more expressed compared to that of prednisolone on both models. Furthermore, C60FAS inhibited pan-cytokeratin expression in HepG2 cells in a dose-dependent manner.

 

CONCLUSION:

Pristine C60 fullerene inhibits liver inflammation and fibrogenesis and partially improved liver and pancreas state under acute and chronic cholangitis.

What is C60 Oil (Fullerene)? + Risks

What is C60?

New Antioxidant Buckminsterfullerene, buckyballs, or C60 is a powerful antioxidant that has effects on unsaturated fats. it removes superoxide, which is a toxic by-product of cellular metabolism that contributes to tissue injury in many human diseases [1].

A review of scientific research concluded that C60 early research holds some promise. The compound had longevity and antioxidant effects in animals and cells. However, it’s still far too soon to draw any conclusions from such limited data [2].

The proposed mechanism indicates that C60 has the ability to acquire positive charge by absorbing protons; this complex could enter the mitochondria, leading to a decrease in reactive oxygen species production [3].

Computer simulations have shown that C60 has the ability to pass through lipid membranes, enter the cell, and alter its functions [4].

Some C60 derivatives that are produced in the body are water-soluble and apparently non-toxic below concentrations of 1 mg/mL [5].

Composition, in vitro antioxidant and antimicrobial activities of essential oil and oleoresins obtained from black cumin seeds (Nigella sativa L.)

Gas chromatography-mass spectrometry (GC-MS) analysis revealed the major components in black cumin essential oils which were thymoquinone (37.6%) followed by p-cymene (31.2%), α-thujene (5.6%), thymohydroquinone (3.4%), and longifolene (2.0%), whereas the oleoresins extracted in different solvents contain linoleic acid as a major component. The antioxidant activity of essential oil and oleoresins was evaluated against linseed oil system at 200 ppm concentration by peroxide value, thiobarbituric acid value, ferric thiocyanate, ferrous ion chelating activity, and 1,1-diphenyl-2-picrylhydrazyl radical scavenging methods. The essential oil and ethyl acetate oleoresin were found to be better than synthetic antioxidants. The total phenol contents (gallic acid equivalents, mg GAE per g) in black cumin essential oil, ethyl acetate, ethanol, and n-hexane oleoresins were calculated as 11.47 ± 0.05, 10.88 ± 0.9, 9.68 ± 0.06, and 8.33 ± 0.01, respectively, by Folin-Ciocalteau method. The essential oil showed up to 90% zone inhibition against Fusarium moniliforme in inverted petri plate method. Using agar well diffusion method for evaluating antibacterial activity, the essential oil was found to be highly effective against Gram-positive bacteria.

A carboxyfullerine SOD mimetic improves cognition and extends the lifespan of mice

In lower organisms, such as Caenorhabditis elegans and Drosophila, many genes identified as key regulators of aging are involved in either detoxification of reactive oxygen species or the cellular response to oxidatively-damaged macromolecules. Transgenic mice have been generated to study these genes in mammalian aging, but have not in general exhibited the expected lifespan extension or beneficial behavioral effects, possibly reflecting compensatory changes during development. We administered a small-molecule synthetic enzyme superoxide dismutase (SOD) mimetic to wild-type (i.e. non-transgenic, non-senescence accelerated) mice starting at middle age. Chronic treatment not only reduced age-associated oxidative stress and mitochondrial radical production, but significantly extended lifespan. Treated mice also exhibited improved performance on the Morris water maze learning and memory task. This is to our knowledge the first demonstration that an administered antioxidant with mitochondrial activity and nervous system penetration not only increases lifespan, but rescues age-related cognitive impairment in mammals. SOD mimetics with such characteristics may provide unique complements to genetic strategies to study the contribution of oxidative processes to nervous system aging.

Extra-Virgin Olive Oil: Super food for pets

Extra-Virgin Olive Oil: Super food for pets

 

What’s the difference between extra-virgin olive oil, and plain olive oil? Does it matter which one I give my pet?

 

Olive oils are graded according to their extraction process and on the acidity of the pressed oil. Extra-virgin olive oil is extracted from olives using pressure only, a process called cold pressing. “Extra-virgin olive oil has 1% acid. It’s the oil that comes from the first pressing of the olives, and is considered the finest, having the freshest, fruitiest flavor,” says Dr. Timothy Harlan, MD, assistant professor of clinical medicine at Tulane University.

 

If we’re committed to feeding our pets nutritious foods, it makes sense to try and deliver the best. 

Olive Oil Offers Unique Cardiovascular Protection By Marsha McCulloch, RD

Olive oil has been pinpointed as a major source of the cardiovascular benefits long associated with a Mediterranean diet.

A recent study of a healthy Mediterranean population showed that olive oil consumption accounted for as much as a 44% reduction in cardiovascular deaths compared to those who didn’t use olive oil.

 

But did you know that if you’re not consuming the right type or amount of olive oil, you might not be getting much benefit at all?

 

This is because different types of olive oil have varying amounts of polyphenols. Although olive oil’s health benefits have historically been attributed to its high monounsaturated fatty acid content, new evidence suggests it’s the polyphenols in olive oil, which have anti-inflammatory properties, that may contribute most to the oil’s cardiovascular benefits.

USA Today: Extra virgin olive oil staves off Alzheimer's, preserves memory, new study shows

USA Today: Extra virgin olive oil staves off Alzheimer's, preserves memory, new study shows

Temple University research shows extra-virgin olive oil protects against memory loss, preserves the ability to learn and reduces conditions associated with Alzheimer's disease

Researchers at the college's Lewis Katz School of Medicine found mice with EVOO-enriched diets had better memories and learning abilities compared to the rodents who didn't eat the oil.

The real effect of EVOO appeared in the inner-workings of the mice's brains. Neuron connections in the brain were better preserved in those on an EVOO diet.

Pristine C60 Fullerenes Inhibit the Rate of Tumor Growth and Metastasis

Pristine C60 Fullerenes Inhibit the Rate of Tumor Growth and Metastasis

S.V. Prylutska1,*, A.P. Burlaka2 , Yu.I. Prylutskyy1 , U. Ritter3 , P. Scharff3

 

Aim: To estimate the impact of C60 fullerene aqueous solution (C60FAS) on the rate of transplanted malignant tumor growth and metastasis. Methods: Lewis lung carcinoma was transplanted into С57Bl/6J male mice. Conventional methods for the evaluation of antitumor and antimetastatic effects have been used. Results: The C60FAS at low single therapeutic dose of 5 mg/kg inhibited the growth of transplanted malignant tumor (antitumor effect) and metastasis (antimetastatic effect): the maximum therapeutic effect was found to be of 76.5% for the tumor growth inhibition; the increase of animal life span by 22% was found; the metastasis inhibition index was estimated as 48%. Conclusion: It was found that water-soluble pristine С60 fullerenes efficiently inhibit the transplanted malignant tumor growth and metastasis.

Sub-acute oral toxicity study withn fullerence C60 in rats

Mika Takahashi1, Hina Kato1, Yuko Doi2, Akihiro Hagiwara2, Mutsuko Hirata-Koizumi1, Atsushi Ono1, Reiji Kubota3, Tetsuji Nishimura3 and Akihiko Hirose1

 

To obtain initial information on the possible repeated-dose oral toxicity of fullerene C60, Crl:CD(SD) rats were administered fullerene C60 by gavage once daily at 0 (vehicle: corn oil), 1, 10, 100, or 1,000 mg/kg/day for 29 days, followed by a 14-day recovery period. No deaths occurred in any groups, and there were no changes from controls in detailed clinical observations, body weights, and food consumption in any treatment groups. Moreover, no treatment-related histopathological changes were found in any organs examined at the end of the administration period and at the end of the recovery period. Blackish feces and black contents of the stomach and large intestine were observed in males and females at 1,000 mg/kg/day in the treatment group. There were no changes from controls in the liver and spleen weights at the end of the administration period, but those weights in males in the 1,000 mg/kg/day group increased at the end of the recovery period. Using liquid chromatography-tandem mass spectrometry, fullerene C60 were not detected in the liver, spleen or kidney at the end of the administration period and also at the end of the recovery period. In conclusion, the present study revealed no toxicological effects of fullerene C60; however, the slight increases in liver and spleen weights after the 14-day recovery period may be because of the influence of fullerene C60 oral administration.

Read research

Update on C60 fullerenes in olive oil | AgingSciences - Anti-Aging Firewalls™

Update on C60 fullerenes in olive oil | AgingSciences - Anti-Aging Firewalls™

By Vince Giuliano and James P Watson

 

More than a year has passed since publication of the November 2012 blog entry Buckyballs, health and longevity – state of knowledge, It will be another year or two before we know whether researchers are capable of reproducing the extraordinary longevity impacts on rats reported in the 2011 publication The prolongation of the lifespan of rats by repeated oral administration of [60] fullerene.

Possible Mechanisms of Fullerene C60 Antioxidant Action

Possible Mechanisms of Fullerene C60 Antioxidant Action

BioMed Research International

 

Novel mechanism of antioxidant activity of buckminsterfullerene C60 based on protons absorbing and mild uncoupling of mitochondrial respiration and phosphorylation was postulated. In the present study we confirm this hypothesis using computer modeling based on Density Functional Theory. Fullerene's geroprotective activity is sufficiently higher than those of the most powerful reactive oxygen species scavengers. We propose here that C60 has an ability to acquire positive charge by absorbing inside several protons and this complex could penetrate into mitochondria. Such a process allows for mild uncoupling of respiration and phosphorylation. This, in turn, leads to the decrease in ROS production.

What is Carbon 60 (C60)

Carbon 60 (C-60) is a Carbon structure based on the Buckminster Fuller Geodesic Dome design (nicknamed Buckyballs). C-60 is also known as Fullerene – after Bucky Fuller. C-60 is a molecule that is shaped very much like a soccer ball. It is a molecule in the shape of a spherical ball, made of 60 carbon atoms (C60). Its structure has both hexagon and pentagon formations. It is found in nature, as the result of the residuals of a lightning strike typically in soot or other carbon-based ash in very small amounts. The C-60 is also made in laboratories with an expensive and time consuming synthetic process. 
In the numerous independent Laboratories and Universities research studies, primarily in Russia, Europe, China, and Japan, noted in the references cited below have shown remarkable health and longevity benefits. The medical and chemical research industry noticed the physical properties of C-60 several years ago in the mid-1980’s. They discovered that C-60 was physically inactive, in that they won’t react with anything indicating that they are harmless and totally non-toxic to the body.  
C-60 atoms are chemically inert and the spherical ball shape forms a perfectly symmetrical structure… at the very small Nano level, as in Nano-technology. No other molecule has been found in the current research that bonds with C-60 thus allowing unstable molecules, such as free radicals, to adhere to the C-60 molecules. This permits the C-60 to remove the free radicals as a very superior antioxidant – research shows it is 100 times more effective in removing free radicals than Vitamin E and 172 times more effective than Vitamin C.
Since C-60 molecules will not dissolve in water and are chemically inert, C-60 research has discovered organic oil that will, with a specific mixing process, act as a carrier of the C-60 – specifically Olive Oil, Coconut Oil, Avocado Oil as well as several others. After testing 9 different organic oils, such as Grape Seed Oil, Apricot Oil, Linseed Oil, etc., the research found Olive to be superior.  In addition, Olive Oil is readily available and less expensive than the other oils. 
C-60 is the combination of C-60 at the purity level of 99.95% and organic, cold pressed pure Olive Oil, Coconut Oil or Avocado Oil. C-6Olive is the product of a proprietary process that combines the two ingredients, as well as a proprietary frequency enhancement process into the final version that is marketed by Live Longer Labs.


Among the documented benefits reported in the research and testing are:
•    Longevity – Research Shows a Doubling or more of Life Expectancy.
•    Inhibiting the Rate of Tumor Growth and Metastasis
•    Superior Antioxidant 
•    Cardiovascular Health
•    Improved Cognitive Ability  
•    Increased Stamina  & Overall Feeling Good  

The prolongation of the lifespan of rats by repeated oral administration of [60]fullerene

The prolongation of the lifespan of rats by repeated oral administration of [60]fullerene

SciVerse ScienceDirect and Elsevier.com

 

 

Countless studies showed that [60]fullerene (C(60)) and derivatives could have many potential biomedical applications. However, while several independent research groups showed that C(60) has no acute or sub-acute toxicity in various experimental models, more than 25 years after its discovery the in vivo fate and the chronic effects of this fullerene remain unknown. If the potential of C(60) and derivatives in the biomedical field have to be fulfilled these issues must be addressed. Here we show that oral administration of C(60) dissolved in olive oil (0.8 mg/ml) at reiterated doses (1.7 mg/kg of body weight) to rats not only does not entail chronic toxicity but it almost doubles their lifespan. The effects of C(60)-olive oil solutions in an experimental model of CCl(4) intoxication in rat strongly suggest that the effect on lifespan is mainly due to the attenuation of age-associated increases in oxidative stress. Pharmacokinetic studies show that dissolved C(60) is absorbed by the gastro-intestinal tract and eliminated in a few tens of hours. These results of importance in the fields of medicine and toxicology should open the way for the many possible -and waited for- biomedical applications of C(60) including cancer therapy, neurodegenerative disorders, and ageing.
The prolongation of the lifespan of rats by repeated oral administration of [60]fullerene (PDF Download Available). Available from: https://www.researchgate.net/publication/224004891_The_prolongation_of_the_lifespan_of_rats_by_repeated_oral_administration_of_60fullerene [accessed Sep 1, 2017].

Olive Oil Offers Unique Cardiovascular Protection

Olive Oil Offers Unique Cardiovascular Protection

By Marsha McCulloch, RD

Olive oil has been pinpointed as a major source of the cardiovascular benefits long associated with a Mediterranean diet.1,2 A recent study of a healthy Mediterranean population showed that olive oil consumption accounted for as much as a 44% reduction in cardiovascular deaths compared to those who didn’t use olive oil.3